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KMID : 0861020080230030019
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Korea Journal of Herbology
2008 Volume.23 No. 3 p.19 ~ p.25
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Protective Effects of Thujae Semen against Neurotoxicity Induced by 6-hydroxydopamine in PC12 Cells
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Kim Hyo-Geun
Shim Jin-Sup
Ju Mi-Sun
Cho Seung-Hun
Oh Myung-Sook
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Abstract
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Objectives : This study was performed to evaluate the neuroprotective effect of water extracts from Thujae Semen(TSW) in PC12 cells.
Methods : We performed 2,2-diphenyl-2-picrylhydrazyl(DPPH) radical scavenging assay, 2,2-azinobis-(3-ethyl-benzothiazoline-6-sulfonic acid(ABTS) cation scavenging assay, and determination of total polyphenolic content to examine the antioxidant effects of TSW. We also carried out 3-(4,5- dimethylthiazol-2-yl)-2,5-diphenyl-tetrazolium bromide assay(MTT), reactve oxygen species(ROS) assay, and nitric oxide(NO) assay to examine neuroprotective effects against 6-hydroxydopamine(6-OHDA) in PC12 cells.
Results : TSW showed IC50 values of 404.3 and 219.9 μg/mL in DPPH and in ABTS assays, respectively. TSW showed 9.74 μg/mg of total polyphenol contents. TSW incresed cell viability in a dose dependent manner and it showed protective effect against 6-OHDA neurotoxicity at the concentration of 25-200 μg/mL. Moreover, it recovered 6-OHDA induced cell death at the same concentrations. The extract showed a dose dependent reduction of ROS and NO generation by 6-OHDA.
Conclusions : We concluded that TSW has neuroprotective effect against 6-OHDA-induced toxicity in PC12 cells through ROS and NO inhibition.
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KEYWORD
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Thujae Semen, PC12, 6-OHDA, neuroprotection
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